Identification and Molecular Characterization of Inducible Immune Protein in Bombyx mori L
سال انتشار: 1402
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 118
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شناسه ملی سند علمی:
JR_JABR-10-1_006
تاریخ نمایه سازی: 6 تیر 1402
چکیده مقاله:
Introduction: In insect immunity, antibacterial proteins are an important part of the immune system. These proteins are mostly produced by epithelial cells and released through hemolymph. Antibacterial proteins in insects belong to the five major families of cecropins, defensins, attacinlike proteins, proline-rich peptides, and lysozymes. Considering the importance of these proteins in fighting infections, the aim of this study was to evaluate and identify these proteins in silkworms (Bombyx mori).Materials and Methods: In the present work, profiling of the proteins present in the hemolymph of control silkworms versus, those infected with bacteria was performed by SDS-PAGE, ۲D gel electrophoresis, and image analysis. We also used MALDI-TOF and MS/MS to investigate novel and uncharacterized immune protein. For this aim, the silkworm hemolymph after inoculation and infection with Staphylococcus aureus bacteria was analyzed using SDS-PAGE, ۲-dimensional gel electrophoresis, MALDI-TOF and MS/MS to identify the immune proteins. The Swiss-Prot and NCBI databases were used for protein identification.Results: A novel protein with a molecular weight of ۳۱.۹ KDa was discovered on the fourth day of exposure to bacteria. The expressed protein showed effective activity against S. aureus, which was infected silkworms. In the MALDI-TOF/MS result and protein identification analysis, ۹۰ numbers of mass values were searched, the mass values matched ۱۶, the total sequence coverage was ۴۶%, and their score was ۵۷. According to the analyses, the expressed protein belongs to the hemolysin secretion protein (HlyD).Conclusions: The results led to the identification of a new protein with antimicrobial properties in silkworm, although more information is needed.
کلیدواژه ها:
نویسندگان
Mallikarjuna Gadwala
Silkworm Pathology Section, Central Sericultural Research and Training Institute, Mysuru, India
Neetha Kari
Department of Zoology, Karnatak University, Dharwad, India
Bangeppagari Manjunatha
Department of Cell Biology and Molecular Genetics, Sri Devaraj Urs Academy of Higher Education and Research, Sri Devaraj Urs Medical College, Kolar, Karnataka, ۵۶۳ ۱۰۳, India
Manthira Moorthi
Central Silk Board, Bangalore, India
Shyam Kumar Vootla
Department of Biotechnology and Microbiology, Karnatak University, Dharwad, India