Study the Effect of PH on Absorption and Fluorescence Spectra of Eosin Y Dye in Ethanol
محل انتشار: نشریه آسیایی شیمی سبز، دوره: 7، شماره: 3
سال انتشار: 1402
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 203
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شناسه ملی سند علمی:
JR_AJGC-7-3_002
تاریخ نمایه سازی: 27 خرداد 1402
چکیده مقاله:
The excitation wavelength (λex= ۴۹۰ nm) at room temperature was determined to examine the impact of pH on the absorption and emission spectra of an eosin y compound dissolved in ethanol at a concentration of ۱×۱۰-۵ M. The relative fluorescence intensity of eosin in ethanol at the concentration of ۱×۱۰-۵ M was decreasing with the increasing of acidity (pH < ۷) and the red shift of fluorescence was decreasing by adding a few drops of ۱N solution of HCl, (pH normal = ۷.۲۶). However when a few droplets of NaOH were added, it was observed that the opposite, the relative fluorescence intensity was increasing with increasing basicity (pH > ۷) and the red shift of fluorescence was decreasing with increasing basicity. Characteristics like fluorescence and absorption wavelength were further measured, along with graphical correlations like the one between pH and the relative fluorescence intensity. The relative fluorescence intensity of eosin in ethanol at the concentration of ۱×۱۰-۵ M was decreasing with increasing acidity and the red shift of fluorescence was decreasing with increasing acidity (pH ۷). However when a few droplets of NaOH were added, it was observed that the opposite was true: the relative fluorescence intensity was increasing with increasing basicity and the red shift of fluorescence was decreasing with increasing basicity.
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نویسندگان
Waleed Khalid Hamood Al-Behadili
Department of Physiology, College of Medicine, Misan University, Iraq
Yaqoob M. Jawad
Department of Physics, College of Science, University of Diyala, Iraq
Hasan J. Hasan
Sumer University, Faculty of Basic Education, University of Kufa, Kufa, Iraq
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