Neuroprotective role of Nesfatin-۱ via modulating level of IL-۱βafter cerebral ischemia in rat

Introduction and Background: Interleukin-۱ beta (IL-۱β) is a key contributor to ischemic brain injuryand it is response increases permeability of the blood–brain, such that exacerbates neuronal cell death andneurological deficits. Brain ischemia-reperfusion (I/R) induces irreversible damages, especially in thehippocampus area. Nesfatin-۱, a recently discovered peptide, protects dopaminergic cells againstneurotoxicity with the anti-inflammatory and anti-apoptotic mechanisms. This study was designed for thefirst time to investigate the protective effects of Nesfatin-۱ on the Interleukin-۱ beta (IL-۱β) protein levelin the hippocampus area in an experimental model of transient global cerebral ischemia.Material and Methods: The male Wistar rats were randomly allocated into ۴ groups (sham, Nesfatin-۱,ischemia-reperfusion, and ischemia-reperfusion+ Nesfatin-۱) (n =۷). The model of cerebral ischemia wasprepared by common carotid arteries occlusion for ۲۰ minutes. Nesfatin-۱ (۲۰ μg/kg) and saline (as avehicle) were injected (intraperitoneally) at the beginning of the reperfusion period. The assessment of theIL-۱β protein level in the hippocampus area was performed by Enzyme-linked immunosorbent assay(ELISA).Results: Nesfatin-۱ significantly reduced the level of IL-۱β in the hippocampal area of ischemic ratstreated by Nesfatin-۱, after ischemia that increased in the ischemia group compared to the sham group.Since the IL-۱β is a proinflammatory cytokine that has been identified as an important mediator ofneuronal cell death in cerebral ischemia.Conclusions:The result demonstrated that nesfatin-۱ might suppress I/R-induced neuroinflammation viareduction IL-۱β protein level. That is the time for a hard outcomes trial of inhibition IL-۱β activation byNesfatin-۱ in cerebral ischemia can be promise a new treatment way.

کلیدواژه ها:

Nesfatin-۱ ، cerebral ischemia ، hippocampus ، Interleukin-۱ beta