Modulatory roles of ergothioneine on heat shock protein-۷۰, tumour necrosis factor-alpha, and rectal temperatures of Arabian stallions following race of ۲۰۰۰ m in a hot-dry environment

Experiments were performed to determine the effect of ergothioneine on rectal temperature and the serum concentrations of heat shock protein-۷۰ (HSP-۷۰) and tumor necrosis factor-α (TNF-α) in stallions following a race of ۲۰۰۰ m in a hot-dry environment. Eighteen stallions weighing approximately ۴۰۰ kg each were used for the experiment. They were divided into three groups of six stallions each. Group I (EEX) was the experimental group that was administered ergothioneine (۰.۵ mg/kg per os), while group II (EEC) did not receive ergothioneine before exercise. The third group (EEN) was neither administered ergothioneine nor exercised. The dry-bulb temperature and the relative humidity of the experiment were determined for six days and on the day of the experiment. The temperature-humidity index was also calculated. Rectal temperature, serum HSP-۷۰, and TNF-α concentrations of all horses were measured before commencement, immediately after, and ۲ h after the exercise. The dry-bulb temperature and relative humidity which showed diurnal fluctuations increased significantly (p < ۰.۰۵) between ۰۶.۰۰ h and ۱۲.۰۰ h (۲۲.۶ ± ۱.۲۳ and ۳۸.۶ ± ۶.۵, respectively). Serum TNF-α and HSP-۷۰ levels of the stallions in the EEX group were higher than the values obtained in the EEC and EEN groups (p < ۰.۰۵). The values of rectal temperature obtained were lower (p < ۰.۰۵) in the EEX group than in the other groups. Therefore, it could be concluded that ergothioneine modulated rectal temperature, as well as TNF-α and HSP-۷۰ concentrations in the stallions, and might be beneficial to horses during exercise.

کلیدواژه ها:

Ergothioneine ، Hot-Dry Season ، tumor necrosis factor-α ، Heat shock protein-۷۰ ، Rectal temperature