Safety of olibanum as food additive in mice gestation period.(“Effect of olibanum on bone ossification process”)

Objective: Frankincense is one of the medicinal plants which has been used in the treatment of a wide variety of diseases. One of the main traditional uses of this plant is in women gestation period in order to have smarter child. Since the most evident action of this plant is the inhibition of ۵- lipooxygenase enzyme and no investigation was done by the regard to its probable following effect on bone mass, so this study was aimed to study safety of olibanum usage in femur in mice whose mothers exposed to it in their gestation period.Materials & methods: A total of ۹۰ BALB/C pregnant mice were used divided into ۵ experimental and control groups. Except two groups which are our control groups, three experimental groups received intraperitoneal injection of olibanum extract (۵۰۰ – ۱۰۰۰ – ۱۵۰۰ mg/kg) daily on gestational days (GDs) ۶ to ۱۵. H&E staining was used for microscopic studies and some bone parameters are evaluated in ۱۶th day embryos and also in mice after one and thirty days after births.Results: Analyzes of microscopic parameters was shown that datas in the group which received olibanum extract by the dose of ۱۰۰۰ mg/kg were statistically significant when compared.Conclusion : This study has documented that intraperitoneal usage of the olibanum by the dose of ۱۰۰۰ mg/kg during pregnancy can cause to accelerate bone growth and also histological changes in ossification process.

کلیدواژه ها:

Frankincense ، olibanum ، H&E staining ، Femur.