Performance and accuracy of SD Bioline malaria Ag P.f (۰۵fk۵۰) in relation to microscopy as the gold standard technique for malaria diagnosis

Successful malaria diagnosis is the mainstay of successful treatment, prevention and eradication of malaria infection. Apart from the gold standard technique (Microscopy), numerous diagnostic techniques perform a similar function to microscopy and in most cases tend to have varying sensitivity and specificity, especially when compared with the gold standard technique. Therefore this study aimed to determine the Performance and accuracy of SD Bioline Malaria Ag P.f (۰۵fk۵۰) (Rapid Diagnostic Test kit) to Gold standard (Microscopy). A total of two hundred (۲۰۰) samples were collected from the consented study subjects and analyzed using RDT and Giemsa staining technique. The result revealed an overall prevalence of ۱۳۲(۶۶.۰%) and ۱۶۷(۸۳.۵%) respectively by RDT and Microscopy, where ۱۱۵ (۵۷.۵%) were true positive, there was no significant difference between the two techniques (P> ۰.۰۵, df= ۱, χ۲ = ۳.۶۹۵). The RDT recorded a sensitivity and specificity value of ۶۸.۸۶% and ۴۸.۴۸% respectively with a positive predictive value of ۸۷.۷۸% and a negative predictive value of ۲۳.۵۳%. The RDT recorded an overall accuracy of ۰.۶۶. The Rapid Diagnostic test kit used in the present demonstrated a high level of sensitivity and positive predictive value with relatively low specificity and negative predictive value. Regular checks on the Performance and accuracy of all brands of RDT should be conducted as their perform

کلیدواژه ها:

Isothermal Amplification ، Loop-Mediated Isothermal Amplification ، Nucleic Acid Amplification Techniques ، P. falciparum ، P. vivax ، P.knowlesi