Ex-vivo study of Aqueous Extract of Nigella Sativa Ag-NPs on Pro-inflammatory Cytokine Gene expression

Osteoarthritis۱ (OA) has been considered a prototypical non-inflammatory arthropathy because neutrophils are absent in the synovial fluid, as are systemic manifestations of inflammation. The involvement of an inflammatory component, which is marked by symptoms such as joint pain, swelling and stiffness, is now well recognized. Inflammatory cytokines, chemokines, and other inflammatory mediators are produced by the synovium and chondrocytes, and can be measured in the synovial fluids of osteoarthritis patients. Nanotechnologies are new areas of research focusing on affecting matter at the atomic and molecular levels. It is beyond doubt that modern medicine can benefit greatly from it; thus nanomedicine has become one of the main branches of nanotechnological research. Therefore, the aim of this study was to evaluate the effect of Extract of Nigella Sativa Ag-NPs (ENS-Ag-NPs)۲ on gene expression of TNF-a۳, iL-۱β۴, COX-۲ ۵, and iNO۶ in bovine fibroblast-like synoviocytes (BFLSCc)۷ and production of PGE۲۸ and NO۹ in THP-۱.Biosynthesis of silver nanoparticles was performed using aqueous extract of Nigella Sativa seed and was characterized by XRD۱۰, FESEM ۱۱ and TEM۱۲ imaging methods. The toxicity of ENS-Ag-NPs was determined by LDH assay and Trypan Blue methods. BFLSc and THP-۱ cells were first incubated with LPS to induce inflammation. Then, the expression of il۱-β, il-۶, iNOS, TNF-α and COX-۲ and production of PGE۲ and No were evaluated using RT-PCR and ELISA respectively. Further, the ENS-Ag-NPs were evaluated for anti-inflammatory activity by ex-vivo method. The results showed ENS-Ag-NPs downregulated TNF-α, iL-۱β, COX-۲, and iNOS expression in synoviocytes this suppression was paralleled by a significant reduction in PGE۲ and NO, respectively, in the cellular supernatant of THP-۱.

کلیدواژه ها:

Pro-inflammatory Cytokine ، Osteoarthritis ، Nigella Sativa ، Silver nanoparticles ، gene Expression