Probiotic Bifidobacterium Lactis Bacteria Inhibit the Invasion Phenotype of Shigella Dysenteriae Induced By Invasion Plasmid Antigen C
سال انتشار: 1398
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 236
فایل این مقاله در 8 صفحه با فرمت PDF قابل دریافت می باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_JIML-6-4_003
تاریخ نمایه سازی: 7 اسفند 1401
چکیده مقاله:
Background and Aims: Shigellosis is an acute gastroenteritis and Invasion plasmid antigen C (IpaC) is the first effector protein for Shigella invasion of intestinal cells. Among lactic acid bacteria, Bifidobacterium lactis (B. lactis) has received increasing attention for protection of a potential host against gastrointestinal infections. The aim of this study was to investigate the inhibitory activity of B. lactis against Shigella dysenteriae (S. dysenteriae) harboring IpaC gene strains from clinical specimens.
Materials and Methods: Sixty stool samples of patients with bloody diarrhea were collected from three teaching hospitals in Tehran and subjected to further analysis for the identification of Shigella colonies by biochemical tests. DNA was assessed for IpaC gene by polymeraese chain reaction (PCR). Furthermore, IpaC gene expression analysis by real-time PCR was carried out to investigate whether probiotic B. lactis can inhibit the invasion phenotype of S. dysenteriae induced by IpaC.
Results: Analysis of gene expression in S. dysenteriae harboring IpaC gene strains showed the expression of IpaC gene in treated S. dysentery with B. lactis being much lower than that of non-treated group (p<۰.۰۰۰). The results revealed that B. lactis at the concentration of ۵۰۰ μg/ml bears strong inhibitory activity on the growth of S. dysenteriae by decreasing IpaC expression.
Conclusions: Our results revealed the positive role of B. lactis in reducing the expression of the ipaC gene and inhibition of epithelial cell invasion by S. dysenteriae. Therefore, probiotics can be used as a complementary biotherapeutic agent in severe Shigella infection.
کلیدواژه ها:
نویسندگان
مریم سادات میرفارسی
Department of Microbiology, Faculty of Biosciences, Islamic Azad University, Tehran North Branch, Tehran, Iran.
صدیقه مهرابیان
Department of Microbiology, Faculty of Biosciences, Islamic Azad University, Tehran North Branch, Tehran, Iran.
الهام سیاسی تربتی
Department of Microbiology, Faculty of Biosciences, Islamic Azad University, Tehran North Branch, Tehran, Iran.
مراجع و منابع این مقاله:
لیست زیر مراجع و منابع استفاده شده در این مقاله را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود مقاله لینک شده اند :