Plasmid Profile and Characterization of Escherichia coli Isolated from Diabetic Foot Ulcers in Imo State, South Eastern Nigeria

سال انتشار: 1401
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 277

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شناسه ملی سند علمی:

JR_JHEHP-8-3_001

تاریخ نمایه سازی: 18 بهمن 1401

چکیده مقاله:

Background: The study aimed at evaluating the plasmid profile and characterization of E. coli isolated from chronic diabetic foot ulcers among patients in Imo State, South East Nigeria. Methods: A total of ۱۵۰ wound swab samples were collected and analyzed. Standard microbiological methods processed samples. Antibiogram was done by Kirby-Bauer disc diffusion method. Tests were conducted to determine biofilm potential, ESBL and AmpC production. Plasmid profiling was done by the alkaline lysis method and Gel electrophoresis. Results: Based on the results, out of the ۱۵۰ swab samples, ۶۱(۴۰.۶%) E. coli isolates were recovered. ۵۷.۸%, ۳۲.۸%, and ۸.۲% of the E. coli isolates were positive for biofilm formation, ESBL, and AmpC production, respectively. Further, the study revealed a pattern of multiple-drug resistance of E. coli to amoxicillin/clavulanic acid (۹۶.۷%), septrin (۹۵.۱%), and ampicillin (۹۳.۴%). Conclusion: Analysis of the multiple antibiotic resistance (MAR) index showed that the organism had MAR index values above the low-risk region (≤۰.۲) for all the tested antibiotics. Post-curring antibiogram tests revealed that nine isolates carried plasmids, suggesting that the mode of resistance may be plasmid-mediated. Findings could act as a therapeutic guide for antibiotic prescriptions in this environment.

نویسندگان

Emmanuel Onwubiko Nwankwo

Department of Microbiology, College Of Natural Sciences, Michael Okpara University of Agriculture, Umudike. P. M. B ۷۲۶۷, Umuahia, Abia State, Nigeria.

Enyinnaya Nwagbara

Department of Medical Laboratory Services, Savealife Mission Hospital, Onitsha, Anambra state, Nigeria.

Ebubechi Uloma Okey-kalu

Department of Microbiology, College Of Natural Sciences, Michael Okpara University of Agriculture, Umudike. P. M. B ۷۲۶۷, Umuahia, Abia State, Nigeria.

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