Schwann cell transplantation exerts neuroprotective roles in rat model of spinal cord injury by combating inflammasome activation and improving motor recovery and remyelination
محل انتشار: هفتمین همایش سالیانه دانشگاه علوم پزشکی سمنان
سال انتشار: 1400
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 107
نسخه کامل این مقاله ارائه نشده است و در دسترس نمی باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
CSUMSMED07_214
تاریخ نمایه سازی: 20 دی 1401
چکیده مقاله:
Background and aim: Inflammasome activation in the traumatic central nervous system (CNS) injuries is responsible for propagation of an inflammatory circuit and neuronal cell death resulting in sensory/motor deficiencies. NLRP۱ and NLRP۳ are known as activators of inflammasome complex in the spinal cord injury (SCI). In this study, cell therapy using Schwann cells (SCs) was applied for targeting NLRP inflammasome complexes outcomes in the motor recovery. These cells were chosen due to their regenerative roles for CNS injuriesMaterials and method: SCs were isolated from sciatic nerves and transplanted to the contusive SCI-induced Wistar rats. NLRP۱ and NLRP۳ inflammasome complexes and their related pro-inflammatory cytokines were assayed in both mRNA and protein levels. Neuronal cell survival (Nissl staining), motor recovery and myelination (Luxol fast blue/LFB) were also evaluated. The groups were laminectomy, SCI, vehicle and treatment. The treatment group received Schwann cells, and the vehicle group received solvent for the cellsResults: SCI caused increased expressions for both NLRP۱ and NLRP۳ inflammasome complexes along with their related pro-inflammatory cytokines, all of which were abrogated after administration of SCs (except for IL-۱۸ protein showing no change to the cell therapy). Motor deficits in the hind limb, neuronal cell death and demyelination were also found in the SCI group, which were counteracted in the treatment group.Conclusion: From our findings we conclude promising role for cell therapy with SCs for targeting axonal demyelination and degeneration possibly through attenuation of the activity for inflammasome complexes and related inflammatory circuit.
کلیدواژه ها:
نویسندگان
Mahboubeh Mousavi
Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
Azim Hedayatpour
Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
Keywan Mortezaee
Department of Anatomy, School of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran
Farid Abolhassani
Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
Gholamreza Hassanzadeh
Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran