A Study of Matrix Metalloproteinase ۹ and CD۳۱ Expression through Immunohistochemistry in Invasive Breast Cancer

Background & Aims: This study was performed to investigate possible relationships between the manifestation of stromal cells (fibroblasts and/or myofibroblasts) by focusing on expression of their matrix metalloproteinase ۹ (MMP۹) and possible angiogenesis based on CD۳۱ and CD۳۴ antigen expression during the various steps of hyperplastic changes to precancerousstate and invasive breast cancer. Methods: Our study included ۵۰ females with invasive ductal carcinoma. Samples were obtained by mastectomy or biopsy and were immunohistochemically stained for the MMP۹, CD۳۱, and CD۳۴ antibody. microvessel count (MVC) was also carried out on samples. Statistical analysis of the data was performed using ANOVA and Student's t-test (P < ۰.۰۵). Findings were compared with our "Breast Cancer Data Bank" for reevaluation of their clinical staging. Results: Positive significant correlations were observed between expression of MMP۹ and invasive ductal carcinoma in situ (DCIS) and fibrocystic disease ± ductal intraepithelial neoplasia (FCD ± DIN) areas (P=۰.۰۰۱). MMP۹ expression in invasive areas was more strongly positive than precancerous areas. Statistically significant correlations were observed between MMP۹ expression and CD۳۱ in grade II in invasive areas. MVC was evaluated by CD۳۱ antibody. It was found to be inversely related to increased MVC from invasive areas, DCIS, DIN, and normal areas (P<۰.۰۰۱). No significant difference was observed in MVC based on age, tumor size or steroid receptors in stroma of an invasive cancer, DCIS, and FCD ± DIN. Conclusion: MMP۹ expression in invasive areas was more strongly positive than precancerous areas, and negative in normal areas. Angiogenesis can be observed before any significant changes in preinvasive breast lesions. The elevated content of microvessel count of the tumor may be an indicator for worse prognostic factor. The progression from epithelial hyperplasia toward DCIS, and then, invasive carcinoma seems too complex to be assumed a linear progression

کلیدواژه ها:

Invasive breast carcinoma ، Ductal carcinoma in situ (DCIS) ، Ductal intraepithelial neoplasia (DIN) ، Matrix metalloproteinase ۹ (MMP۹) ، CD۳۱