Evaluation of lncRNA FOXD۲-AS۱ Expression as a Diagnostic Biomarker in Colorectal Cancer

Background: Colorectal cancer (CRC) is still considered one of the prevalent cancers worldwide. Investigation of potential biomarkers for early detection of CRC is essential for the effective management of patients using therapeutic strategies. Considering that, this study was aimed to examine the changes in lncRNA FOXD۲-AS۱ expression through colorectal tumorigenesis. Methods: Fifty CRC tumor tissues and fifty adjacent normal tissue samples were prepared and involved in the current study. Total RNA was extracted from the samples and then reverse transcribed to complementary DNA. Next, the expression levels of lncRNA FOXD۲-AS۱ were evaluated using real-time PCR in CRC samples compared to normal ones. Also, receiver operating characteristic curve analysis was used to evaluate the diagnostic value of FOXD۲-AS۱ for CRC. Results: The obtained results showed that the expression level of FOXD۲-AS۱ gene was significantly (p<۰.۰۰۰۱) up-regulated in tumor tissues compared to normal marginal tissues. Also, a significant correlation was observed between higher the expression of FOXD۲-AS۱and the differentiation of tumor cells. Furthermore, ROC curve analysis estimated an AUC value of ۰.۵۹ for FOXD۲-AS۱, suggesting its potential as a diagnostic target. Conclusions: Taken together, the current study implied that tissue-specific upregulation of lncRNA FOXD۲-AS۱ might be appropriate diagnostic biomarkers for CRC. Nonetheless, more studies are needed to validate these results and further illustrate FOXD۲-AS۱ function through colorectal tumorigenesis.

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Keywords: Biomarker ، Colorectal cancer ، FOXD۲-AS۱ ، lncRNA ، qRT-PC.