Effect of Hydrogel S tiffness on Biological Features ofNormal and Cancer Cells
محل انتشار: بیست و سومین کنگره بین المللی هیبریدی پزشکی تولید مثل و هجدهمین کنگره هیبریدی فناوری سلولهای بنیادی رویان
سال انتشار: 1401
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 114
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شناسه ملی سند علمی:
RROYAN23_307
تاریخ نمایه سازی: 17 دی 1401
چکیده مقاله:
Objective: Cancer is a major challenge in the field of healthcare, which is spreading, especially in developing countries. Althoughdifferent treatments have been developed to cure it, cancercells are eventually able to resis t agains t these treatments.During tumorigenesis, the interaction between cancer cells andthe tumor micro-environment often leads to ECM s tiffness.Therefore, the aim of this s tudy is to inves tigate the effect ofs tiffness as an important mechanical parameter of ECM on theproliferation and migration of normal and cancer cells.Materials and Methods: We used alginate as a natural hydrogeldue to its desirable properties such as biocompatibility, easeof gelling, low biodegradability, negative electrical charge, andbio-inert nature to encapsulate the cells. Initially, calcium alginatebeads were prepared with two different molecular weightsof alginate, and their hydrolytic s tability was s tudied in PBS.After selecting the medium molecular weight as the appropriateoption, the calcium alginate beads with different s tiffness contentsof ۰.۷ - ۴۳.۵ kPa, were prepared through controlling thegelation time and concentration of the crosslinker.Results: Finally, the effect of hydrogels’ s tiffness on proliferationand migration of both normal and cancer fibroblas t cellswas probed by the cell encapsulation process. Both MTT andscratch assay results showed that both normal and cancer cellsshowed a good responsivity to the s tiffness of the hydrogels.Conclusion: Our results well showed that both molecularweight and s tiffness can affect the proliferation and migrationof normal and cancer cells.
کلیدواژه ها:
نویسندگان
P Yousefi
Farzanegan ۳ High-School, Karaj, Iran
H Mohajer
Farzanegan ۳ High-School, Karaj, Iran
M Zare Gachi
Department of Biomaterials, Zharfandishan Fanavar Zis tbaspar(ZFZ) Chemical Company, Tehran, Iran
H Daemi
Department of Cell Engineering, Cell Science Research Center,Royan Ins titute for S tem Cell Biology and Technology, ACECR,Tehran, Iran
M Mashayekhi
Department of Biomaterials, Zharfandishan Fanavar Zis tbaspar(ZFZ) Chemical Company, Tehran, Iran