The Small Molecule Enoxacin Prohibits The In VivoGrowth and Tumorigenicity of Esophageal Cancer Cells

Objective: Esophageal carcinoma (EC) is one of the mos t lethalcancers worldwide. Several cancer types, including EC, showglobal downregulation of microRNAs, i.e., non-coding RNAsinvolved in pos t-transcriptional gene regulation, to enable higherinvasiveness. Therefore, we aimed to analyze if enoxacin, amicroRNA-enhancing small molecule, could inhibit EC growth in vitro and in vivo.Materials and Methods: To inves tigate enoxacin (۱۲۴ μM) inEC cells (KYSE-۳۰ line) in vitro, MTT viability assay, Crys talViolet s taining, and Live/Dead assay were performed ۷۲ hourspos t-treatment. The migratory ability of the cells was carriedout using a wound healing assay. A sub-cutaneous mouse modelof EC was es tablished using KYSE-۳۰ and immunocompromisedmice. The cells (۴×۱۰۶) were injected into nude mice۷۲ hours after treatment with enoxacin (۱ mg/kg). Tumor tissueswere removed and fixed in ۱۰% buffered formalin. Next,paraffin-embedded specimens were cut into serial sections ands tained with hematoxylin and eosin. Data were analyzed usingGraphPad Prism and ImageJ (P value <۰.۰۵).Results: We found that enoxacin reduced the growth and viabilityof EC cells in vitro. We also observed that enoxacin wasable to res trict the migration of the cells. Furthermore, the tumorformation ability of EC cells in mouse models was decreased inenoxacin-treated cells compared to the control groups (n = ۵).Particularly, no tumors (n = ۴) or only small tumors (n = ۱) wereobserved in treated mice, judging from his tological evaluations.Finally, the tumors in the treated groups were less aggressivethan the control group.Conclusion: Enoxacin inhibits the growth and migration of ECcells in vitro and in animal models of EC

کلیدواژه ها:

Carcinogenesis ، Esophagus ، Esophageal SquamousCell Carcinoma ، miRNA Pathway ، RNAi