Fabrication and Evaluation of Conductive Polyaniline-Based Nanofibrous Scaffold for Skin Tissue Engineering

Objective: One of the newes t approaches to skin tissue regenerationis designing a scaffold with similar properties to thenatural skin extracellular matrix. Conductive nanofibers can actas multifunctional scaffolds and their electrical nature can s timulatethe proliferation of s tem cells and increase the improvementof the wound healing process. In this s tudy, a polyaniline(PANI) nanofibrous scaffold with a novel dopant was fabricatedfor enhancing skin tissue regeneration.Materials and Methods: Nanofibrous scaffolds were fabricated through electrospinning. PLLA and PANI: dopant (۱:۱) weredissolved in chloroform and dimethylformamide, respectively.Then the solutions were mixed. Brilliant blue (BB) was used asan antibacterial dopant. Electrospinning was carried out withthe high voltage power supply set at ۳۰ kV, an air gap dis tanceof ۳۰ cm, and a flow rate of ۰.۰۰۲ ml/h. The nanofibers werecharacterized using scanning electron microscopy (SEM), attenuatedtotal reflection-Fourier-transform infrared spectroscopy(ATR-FTIR), and four-point-probe analysis. BB releasingwas inves tigated by UV-visible spectroscopy.Results: The presence of doped PANI in the scaffold was confirmedby ATR-FTIR. The morphology of PLLA/PANI-BB nanofiberswas homogenous and beadless and the conductivity ofthis scaffold was acceptable. The BB showed an initial burs trelease, but after two days it had a sus tain-release profile.Conclusion: The prepared scaffold is suitable for skin tissueengineering due to its nanofibrous morphology and electricalconductivity. Furthermore, antibacterial property of the scaffoldmakes it a good option for wound healing.

کلیدواژه ها:

Conductive Nanofibers ، Dopant ، Electrospinning ، Polyaniline ، Skin Tissue Engineering