Bone Marrow CD۳۴ Positive Cells May Be Suitablefor Collection after Death

Objective: Hematopoietic s tem cells (HSCs) which are characterizedwith CD۳۴+ phenotype, have a pivotal role in bloodcell regeneration. They are located in lowes t hypoxic areas inthe bone marrow niches. This microenvironment protects themfrom DNA damage and excessive proliferation, whereas the oxygenatedarea driving cells out of quiescent s tate into proliferation.Given the resis tance of HSCs to hypoxia, it is reasonableto imagine that they can survive for some time in the absenceof oxygen.Materials and Methods: Here, we evaluated CD۳۴, Bax, Bcl-۲, Bcl-xl, and p۵۳ genes expression after death. Moreover, wees tablished the ex-vivo development of HSCs using SCF, FLT۳,IL-۲, and IL-۱۵ cytokines in culture sys tem.Results: Our finding indicated that although the mos t of thedead person's mononuclear cells were alive and adequately expressedthe CD۳۴ on their surfaces at the firs t day of isolation,the viability and CD۳۴+/Ki-۶۷ expression declined significantlyafter culture process.Conclusion: Taken together, our finding indicated that the viabilityand CD۳۴+ expression was acceptable on day ۰ and couldbe used as a novel method for therapeutic purposes

کلیدواژه ها:

CD۳۴ Marker ، Death Individual ، HematopoieticS tem Cells (HSCs) ، Oxygen Tension