Differentiation of Adipose-Derived MesenchymalS tem Cell (ASC) to Os teoblas t in The Presence of Shilajit/Alginate Combination

Objective: Shilajit, as a herbomineral natural subs tance, hasbeen mos t widely used for the remedy of a numerous of illnesssuch as bone defects in traditional folk medicine sincehundreds of years ago. The aim of the present s tudy was toexplore the effect of Shilajit on the os teogenic differentiationof human(adipose derived mesenchymal s tem cells (ASCs) intwo- and three-dimensional cultures.Materials and Methods: ASCs were isolated and suspended in۱% (w/v) alginate solution with or without Shilajit and seededinto culture plates at a density of ۱۵ × ۱۰۴ cell per ۵۰۰μL /wellfor three-dimensional (۳D) cultures. For two-dimensional (۲D)cultures, a suspension of ASCs was seeded into culture plates ata density of ۱۵ × ۱۰۳ cell/well and treated with ۵۰۰ μg/ml Shilajit.Then, characterization was done using SEM/EDX, ALP (alkalinephosphatase) activity, Alizarin red s taining, and Ramanconfocal microscopy.Results: According to findings, Shilajit/alginate combinationin the presence or absence of os teogenic medium significantlyincreased ALP activity and mineralization, compared to the ۲Dmatched groups at all time points (P value <۰.۰۵). In addition,calcium deposition level was obviously high in Shilajit/ alginatecombination with or without os teogenic medium comparedto ۲D matched groups on days ۱۴ (P value <۰.۰۰۰۱), and ۲۱(P value <۰.۰۰۱and P value <۰.۰۱, respectively). Shilajit couldalso promote os teogenic differentiation of s tem cells with orwithout os teogenic medium in two-dimensional cultures. ButShilajit/alginate combination s tarts os teogenic differentiation ina short period of time.Conclusion: As Shilajit accelerates the differentiation of ASCsinto the os teoblas ts, without changing the physical properties ofthe alginate hydrogel, this combination may pave the way formore promising remedies considering bone defects

کلیدواژه ها:

Alginate ، Mesenchymal S tem Cell ، Os teogenic Differentiation ، Shilajit