Fabrication and Evaluation of Piezoelectric Poly(L-Lactide)-Based Electrospun Scaffold for Bone TissueRegeneration

Objective: Piezoelectric materials are widely used to mimicthe electrophysiological nature of the native bone tissue. Severalnatural and synthetic polymer and ceramic scaffolds withpiezoelectric properties can s timulate the proliferation and differentiationof s tem cells and consequently, improve bone regenerationat the defected site, by recons tructing the local electricalmicroenvironment of bone. In this s tudy, a poly(L-lactide)(PLLA)-based electrospun scaffold was fabricated for enhancingbone tissue regeneration.Materials and Methods: Electrospun scaffolds were fabricatedusing different PLLA/hydroxyapatite ratios. For this purpose,the ceramic component was dispersed in chloroform, andthen the polymer component and dimethylformamide solventwere added to the sys tem to be mixed. The electrospinning wascarried out with a voltage of ۲۰ kV, a dis tance of ۲۰ cm betweenthe collector and the needle, and a flow rate of ۰.۷ ml/h. Thenanofibers were characterized using scanning electron microscopy(SEM) and piezoelectric properties were calculated with ahomemade piezo-tes ter.Results: Electrospun fibers showed a bead-free morphologywith a nanometer scale. Increasing the amount of ceramic componentin electrospun nanocomposite increases the piezoelectricproperty, with the maximum piezoelectric value of ۰.۱۴۵۶mV/N.Conclusion: The fabricated scaffold (PLLA/HA; ۸۰:۲۰) is suitablefor bone tissue engineering by virtue of its nanofibrousmorphology and piezoelectric property. Furthermore, the presenceof hydroxyapatite makes the aforementioned nanocompositea promising candidate for bone regeneration

کلیدواژه ها:

Electrospinning ، Bone Tissue Engineering ، NanocompositeFibers ، Piezoelectric