KIR Positive NK Cells Effectively Target LeukemiaCell Lines

Objective: Acute lymphoid (ALL) and myeloid leukemia(AML) are known to be invasive and highly lethal hematologicalmalignancies. Insufficiency of the present treatments and their diverse side effects lead researchers to find new and moreeffective therapeutic methods. Interes tingly, ongoing effortsto find the bes t approach to optimize natural killer (NK) cellanti-leukemia potential shed light for the successful treatmentof cancer. Mature KIR+NK cells’ ability to remove HLA ClassI deficient cells has been exploited in cancer immunotherapy.Materials and Methods: To precisely address the influence ofcomplex cytokines on NK cells development, cord blood derivedMNCs and CD۳۴+ cells were seeded with different cytokinecombination. NK cell differentiation and their cytotoxicfunction were examined with KIRs/CD۵۶ and CD۱۰۷/IFN expression,respectively.Results: Our finding underlined the importance of KIR expressionin cytotoxic function of NK cells. Taken together, thiss tudy presented an effective in vitro method for the expansionand differentiation of KIR+NK cells using cytokines withoutany feeder cells.Conclusion: The presented culture condition could be helpfulfor the generation of mature and pure NK cells even from limitednumbers of CD۳۴+ cord blood cells and might be used asa novel method to improve the current s tate of cancer therapy

کلیدواژه ها:

Acute Lymphoblas tic Leukemia ، Cord Blood S temCells ، Killer Cell Immunoglobulin-Like Receptors ، NaturalKiller Cells