Cryopreservation of Human Sperm: Comparing theEffects of Vitrification Versus Rapid Freezing on The SpermParameters

Background: Sperm cryopreservation, has a key role for fertilitypreservation in male. Recent s tudies have shown that thequality of sperms is reduced after the freeze-thaw process.Therefore, choosing the optimal method of sperm freezingseems necessary to reduce cryoinjury. The aim of this s tudy wasto compare two cryopreservation methods namely vitrificationand rapid freezing on human sperm parameters.Materials and Methods: Semen samples were collected from۲۰ healthy men who referred to Royan Ins titute. After spermprocessing, each sample was divided into two frozen groups:vitrification and rapid freezing. Motility parameters (the SemenClass Analysis Software, CASA), morphology (Papanicolaous taining), viability (eosin-negrosin s taining) and DNA fragmentation(sperm chromatin s tructure assay, SCSA) of thawedsperm were evaluated for both methods.Results: Our results showed a significant decrease in the spermmotility parameters and viability and a significant increase inDNA fragmentation index in the frozen groups compared tofresh group. Moreover, a significant reduction in sperm totalmotility and viability (P<۰.۰۱) and a significant increase inDNA fragmentation index (P<۰.۰۵) were observed in the vitrificationgroup compared to rapid freezing.Conclusion: In conclusion, our findings showed that rapid freezingis a more suitable method for the preservation of sperm cellquality after the freeze-thaw process compared to vitrification.

کلیدواژه ها:

Cellular Parameters ، Human Sperm Cryopreservation ، Rapid Freezing ، Vitrification