Antioxidant Effects of L-Proline on Human SpermCells during The Freezing-Thawing Process

Background: Sperm cryopreservation is an essential aspect ofmale fertility preservation. However, it is widely reported thatthe cryopreservation process induces detrimental impacts onsperm functions.Materials and Methods: The s tudy included forty normozoospermicmales who received antioxidants (zinc + vitamin C).Each sample was aliquoted to ۴. In aliquots ۱ to ۴, experimentalconcentrations of L-proline (۰, ۱, ۲, ۴ mmol/L) were included inthe freezing medium. Sperm parameters (motility, viability, andmorphology) and sperm chromatin and DNA integrity, includingToluidine blue (TB), Chromomycin A۳ (CMA۳), Anilineblue (AB), and sperm chromatin dispersion (SCD), were assessed.The levels of nitric oxide (NO), reactive oxygen species(ROS), total antioxidant capacity (TAC), and lipid peroxidation(LPO) were measured in the sperm freezing medium.Results: ۲ and ۴ mmol/L of L-proline maintained sperm motilityand viability higher than in the control groups. The MDA,NO, and ROS levels were significantly lower in the ۲ and ۴mmol/L L-proline group compared to the control group. Supplementing۲ and ۴ mmol/L of L-proline improved the TAClevels compared to the control group. ۴ mmol/L of L-prolinesignificantly reduced TB and CMA۳ tes ts compared to the controlgroup. A notable decrease in AB and SCD levels was observedin ۲ and ۴ mmol/L of L-proline groups compared to thecontrol group.Conclusion: L-Proline inclusion in the sperm freezing mediumpreserves sperm quality and chromatin and DNA integrity bymodulating oxidative s tress and enhancing antioxidant levels

کلیدواژه ها:

Antioxidant ، Human Spermatozoa ، L-Proline ، OxidativeS tress ، Sperm Cryopreservation