Qing Fei Hua Xian Decoction ameliorates bleomycin-induced pulmonary fibrosis by suppressing oxidative stress through balancing ACE-AngII-AT۱R/ACE۲-Ang-(۱-۷)-Mas axis

Objective(s): We aimed to investigate the preventative effect of Qing Fei Hua Xian Decoction (QFHXD) against pulmonary fibrosis (PF) and its potential mechanisms. Materials and Methods: Bleomycin (BLM)-induced rats were respectively treated with ۴۱۳.۳, ۸۲۶.۶, and ۱۲۳۹.۹ mg/kg of QFHXD and prednisone for ۲۸ days. The lung tissues of rats were collected on day ۲۸ for histological and western blotting analysis. Results: QFHXD significantly reduced alveolus inflammation, collagen accumulation, and fibrosis deposition in BLM-induced PF rats (P<۰.۰۵). Collagen I and III, vimentin, and α-smooth muscle actin(α-SMA) expression levels were likewise decreased in PF rats treated with QFHXD (P<۰.۰۵). Additionally, QFHXD increased the expression of nuclear factor erythroid ۲-related factor ۲ (Nrf۲) while decreasing NADPH oxidase ۴ (NOX۴) expression (P<۰.۰۵). Furthermore, QFHXD suppressed the PF progression by down-regulating Angiotensin-Converting Enzyme (ACE) -Angiotensin II (AngII) -Angiotensin II Type ۱ Receptor (AT۱R) axis (P<۰.۰۱) and up-regulating Angiotensin-Converting Enzyme ۲ (ACE۲) -Angiotensin-(۱-۷) (Ang-(۱-۷)) -Mas axis (P<۰.۰۵). Conclusion: QFHXD suppressed inflammatory infiltration and PF brought on by BLM in lung tissues through reducing oxidative stress by maintaining the equilibrium of ACE-AngII-AT۱R and ACE۲-Ang-(۱-۷) -Mas axes. This study may provide a novel clinical therapy option for PF.

کلیدواژه ها:

Extracellular matrix ، Lung Diseases ، NADPH oxidases ، Oxidative stress ، Pulmonary Fibrosis ، Renin-angiotensin system