Hydroxymethylfurfural Content and Sugar Profile of Honey Available in Bangladesh Using Validated HPLC-PDA and HPLC-RID

Background: Honey has a lot of reputation because of its supposed medicinal properties. In this study, Hydroxymethylfurfural (HMF), sugars, and Fructose/Glucose ratio of honey in Bangladesh were assessed for adulteration and authenticity evaluation. Methods: Seventy honey samples collected from different districts of Bangladesh were analyzed by High Performance Liquid Chromatography (HPLC) for HMF content and sugar profile. The samples were prepared by using Carrez I and Carrez II prior to injecting into HPLC. The samples were then filtered through syringe filter and taken in ۱.۵ ml vial for injecting into the HPLC system. Results: HMF values were ranging from ۱.۴۱ mg/kg to ۲,۰۶۳.۹۰ mg/kg. The Limit of Detection (LOD) and Limit of Quantification (LOQ) was found ۰.۱۰ mg/kg and ۰.۳۳ mg/kg with R۲=۰.۹۹۹۴. The average values of fructose, glucose, and sucrose were in the range of ۱۴.۷۵-۵۲.۴۴%, ۸.۱۹-۴۲.۶۳%, and ۰.۱۰-۲۱.۱۲%, respectively. From validation parameters, LOD values for fructose, glucose, and sucrose were ۰.۰۰۳, ۰.۰۰۸, and ۰.۰۰۴%, respectively; and LOQ values were ۰.۰۱, ۰.۰۲۸, and ۰.۰۱۵%, respectively with an excellent linearity with R۲ for fructose=۱.۰, glucose=۰.۹۹۹۹, and sucrose=۱.۰. Conclusion: Some samples had higher HMF content which may be due to the storage time was increased and improper processing with high temperature or adulteration by High Fructose Corn Syrup (HFCS), sugar cane syrup, rice syrups or rice molasses. The sugar profiles showed that the most of honey samples were nectar honeys. DOI: ۱۰.۱۸۵۰۲/jfqhc.۹.۳.۱۱۱۵۴

کلیدواژه ها:

Honey ، ۵-Hydroxymethylfurfural ، Chromatography ، High Pressure Liquid ، Fraud ، Sucrose ، Bangladesh