Molecular Epidemiology of AmpC-Producing Klebsiella Pneumoniae Isolated from Perioperative Patients in a Tertiary Hospital
سال انتشار: 1400
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 157
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شناسه ملی سند علمی:
JR_SBMU-6-3_010
تاریخ نمایه سازی: 9 آبان 1401
چکیده مقاله:
Background: Extended-spectrum cephalosporin-resistant organisms are the sources of AmpC- type β-lactamase isolates. AmpC-producing Klebsiella pneumoniae isolates are a major challenge in clinics due to their high rate of antibiotic resistance. This study aimed to detect AmpC β-lactamases in K. pneumoniae isolated from hospitalized patients with a surgical history.Materials and Methods: This cross-sectional study was performed on ۱۲۰ urine catheter samples. After antibiotic susceptibility testing, plasmid-mediated AmpC was detected by double-disc plus combined disc test (DCDT) and three-dimensional extract test (TDET). The genotypic detection of plasmid-mediated AmpC was carried out using multiplex-polymerase chain reaction (PCR).Results: Of ۱۲۰ samples, ۶۰ (۵۰%) instances of K. pneumoniae were isolated. The highest and lowest resistance rates were related to cefotaxime (CTX) (۵۰%) and ciprofloxacin (CIP) (۲۳.۳%), respectively. ۱۸.۳% (n: ۱۱) K. pneumoniae showed an AmpC phenotype in DCDT and TDET tests. AmpC genotyping showed that the prevalence of CIT, DHA, EBC, and ACC genotypes were ۴۷.۱%, ۳۵.۳%, ۲۹.۵%, and ۲۳.۶%, respectively. MOX and FOX genotypes were not identified in the isolates of interest.Conclusion: AmpC β-lactamase identification tests should be considered a routine microbiology workup for gram-negative microorganisms. Multiplex-PCR can identify the plasmid AmpC genotypes.
کلیدواژه ها:
نویسندگان
Roghayeh Gholizadeh Doran Mahalleh
Department of Microbiology, School of Medicine, Kerman Branch, Islamic Azad University, Kerman, Iran
Nadia Kazemi Pour
Department of Microbiology, School of Medicine, Kerman Branch, Islamic Azad University, Kerman, Iran
Farokh Rokhbakhsh-Zamin
Department of Microbiology, School of Medicine, Kerman Branch, Islamic Azad University, Kerman, Iran