Short-Term Effect of Aerobic Exercise and High-Fat Diet and Consumption of Curcumin Extract on Leptin Gene Expression in Liver Tissue in Male Rats

Introduction: Leptin is a protein hormone with a spiral structure similar to cytokines, which is mainly synthesized and released by subcutaneous fat cells in a steady pulsating manner with a peak secretion near midnight. The aim of this study was to compare the short-term effects of aerobic exercise and high-fat diet and curcumin extract on leptin gene expression in the liver tissue of male rats. Methods: Fifty male rat with two-month-old were prepared and divided into five groups: control(G۱), high-fat diet(G۲), curcumin and high-fat diet(G۳), exercise and high-fat diet(G۴), curcumin+exercise+high-fat diet(G۵). Data analysis was compared using one-way analysis of variance. Bonferroni's post hoc test was used to accurately determine the differences between groups. The mean of intra-group differences was also compared using the t-test of the sample. Results: The results showed a significant difference in the average leptin gene expression of subjects among ۵ groups. The results of Bonferroni's post hoc test also showed an increase in the high-fat diet group compared to the control group(P=۰.۰۰۰۱), and a decrease in the high-fat diet + curcumin group(P=۰.۰۰۰۱), the high-fat diet + exercise group(P=۰.۰۰۰۱), and the high-fat diet + exercise + curcumin group compared to the control group(P=۰.۰۰۰۱). Conclusion: Leptin gene is expressed under the influence of short-term exercises and the consumption of curcumin, which is an antioxidant. So that the use of each agent alone causes a decrease or increase in the leptin gene expression in the liver, and the simultaneous use of both factors causes a decrease in the leptin gene expression of these cells in the muscle tissue.

کلیدواژه ها:

Short-term aerobic exercise ، High-fat diet ، Curcumin extract ، Leptin gene expression