The probiotic properties and potential of vaginal lactobacillispp. Isolated from healthy women against some vaginal pathogens

Background and Aim : During the last decade, research in probiotic has progressed considerablyand significant advances have been made in the selection and characterization of specific probioticstrains. The most studied probiotics belong to Lactobacillus genera.Methods : The potential probiotic characteristics of ۹۷۰ Lactobacillus strains separated from thevagina of healthy women were evaluated in terms of some safety features such as antibioticsusceptibility and hemolytic activity; probiotic properties like resistance to acidic conditions andbile salts; antagonistic activity against pathogens; lactic acid production; hydrophobicity, autoaggregation,and co-aggregation abilities; hydrogen peroxide production; and adhesion capacity toHela cells.Results : Among the isolates, five strains were identified as Lacticaseibacillus rhamnosus,Lacticaseibacillus casei, Lactiplantibacillus plantarum, Lactobacillus acidophilus andLactobacillus gasseri, presented acceptable antibiotic susceptibility pattern. Further analysisshowed antimicrobial activity of Lacticaseibacillus culture against various bacterial pathogens andreal-time PCR showed all five strains were able to prevent the colonization of bacterial pathogens.All five selected strains produced organic acids, hydrogen peroxide and were resistant to thespermicide. In addition, they lacked hemolytic activity with the ability of hydrophobicity, autoaggregationand co-aggregation with pathogens.Conclusion : These results suggest that the vaginal microbiome could be a good source for theisolation of probiotics and the strains of this study may be considered as good probioticscandidates.

کلیدواژه ها:

Probiotics ، lactic acid bacteria ، Women ، Vaginal microbiota ، Lactobacillus spp.