Antibacterial Activity of Synthesis of Silver Nanoparticles ofMethanolic Extract of Leaves of Anethum graveolens L. fromTonekabon - Iran

Background and Aim : Currently, considerable attention has been made towards metalnanoparticles due to their unique properties that are different from their bulk metals. They havewide applicability in different areas including biology, chemistry, medicine and optoelectronics.Many methods have been developed for the synthesis of metal NPs and most of these methods arebased on chemical reduction and physical methods which are expensive, toxic and nonenvironmentallyfriendly. At present, there is a growing need to use non-toxic, environmentally,and renewable materials for the synthesis of metal nanoparticles. Thus many attempts have focusedon the use of green synthesis approach. Here a simple biosynthesis approach was applied and silvernanoparticles were synthesized by using Anethum graveolens L. leaves extract and its examinedantioxidant and antimicrobial activity.Methods : In this study, Anethum graveolens L. is one of the members of Apiaceae family.Anethum graveolens leaves collected from Tonekabon city were used to synthesis of silvernanoparticles and evaluation of biological properties. Methanolic extracts were investigated forantibacterial activity against six selected bacterial strains (Bacillus subtilis, Staphylococcus aureus,Pseudomonas aeruginosa, Escherichia coli, Salmonella typhi and Shigella dysenteriae) usingminimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC)methods. Antioxidant activity of methanolic extracts was assessed by DPPH۰ method. Synthesisof silver nanoparticles was evaluated by TEM, FT-IR, UV-Vis and XRD.Results : Our research showed that synthesis of silver nanoparticles using methanolic extracts ofAnethum graveolens L. had antibacterial activity on all of the investigated microorganisms. Theamount of IC۵۰ for silver nanoparticles was (۱۸۱.۸±۴.۶ mg/ml). Synthesis of silver nanoparticlesand pictures captured by TEM showed that nanoparticles were spheroids which were distributeduniformly. The size of particles varied between ۱۰ and ۵۰.Conclusion : Finally it can be concluded that the synthesis of silver nanoparticles of leaves extractof Anethum graveolens L. can be used effectively against certain bacteria causing disease in humanbeings as it is a potent antimicrobial agent

کلیدواژه ها:

Anethum graveolens L. ، Pseudomonas aeruginosa ، Silver Nanoparticles ، DPPH۰Method.