Study of Infection of Broiler Chickens in Guilan Provincewith Fowl cholera by ELISA Method

Background and Aim : Fowl cholera or Pasteurellosis is an acute and highly contagious diseasecaused by Pasteurella multocida a gram-negative coccobacillus in the Pasteurellaceae family in arange of avian species including chickens, turkeys, and other birds. This disease is seen worldwideand can range from acute septicaemia to chronic and localised infections. The broiler flocks ismore sensitive than the laying breed .The morbidity and mortality may be up to ۱۰۰% and It canalso occur in humans which shows the importance of investigating this bacterium .Methods : Total ۱۵۰ blood samples were taken from ۱۵ broiler flocks of Guilan province(randomly ۱۰ samples from each flock). Sampling was done by collecting ۲ to ۳ cc of blood .Thesamples were centrifuged at ۳۰۰۰ rpm for ۵ minutes to completely separate the serum and IndirectELISA was performed using by Pasteurella Multocida Antibody Test Kit IDEXX to determine theantibodies titres against Pasteurella Multocida.Results : In this study ۱۵۰ blood samples were collected from broilers in Guilan province and afterexamining the titer of each sample, it was found that ۱۰۰% samples were positive that indicateshigh infection in this flocks.Conclusion : This study was carried out to determine antibodies against Pasteurella Multocida inbroiler flocks of Guilan province. Since all the studied samples had positive titers and it could bedue to high humidity in Guilan province and high pollution of local poultry and non-compliancewith health standards in broiler farms.Because of this, it is recommended that poultry workersshould not have contact with native birds and health standards in broiler flocks should be raised.Also similar research in other provinces with similar and different climates is suggested to bedone with serological and molecular methods.

کلیدواژه ها:

Fowl Cholera ، Pasteurella Multocida Antibody Test Kit IDEXX ، ELISA ، Guilanprovience.