Identification of Infectious Laryngotracheitis antibodies inbroiler flocks in ce Guilan provin

Background and Aim : Infectious laryngotracheitis (ILT) is a respiratory tract Infectious ofchickens caused by Gallid herpesvirus type ۱ (GaHV-۱).This virus infects the upper respiratoryand ocular organ of poultry and characterized by respiratory depression, dyspnea , cough, openmouth breathing and spitting blood exudate from the respiratory tract with high rates of morbidityand mortality.Methods : In this study, ۱۵۰ blood samples were collected from ۱۵ broiler farms, so that ۱۰samples were taken randomly from each farm of Guilan province. Sampling was done bycollecting ۲ to ۳ cc of blood. In the laboratory, the samples were centrifuged at ۳۰۰۰ rpm for ۵minutes to completely separate the serum and were evaluated by synbiotics ELISA kit. It shouldbe noted that all the steps of ELISA test were performed according to the method and instructionsof the kit manufacturer.Results : In this study, blood samples were collected from ۱۵۰ broilers in Guilan province. Afterseparating the serum and examining the titer of each sample, it was found that all samples werenegative.Conclusion : This study was carried out to determine the seroprevalence of InfectiousLaryngotracheitis virus (ILTV) in broiler flocks of Guilan province in Iran. Indirect ELISA wasperformed to determine the antibody titre against ILTV in broiler flocks. Total ۱۵۰ blood sampleswere taken from ۱۵ broiler farms.The serum prevalence of infectious laryngotracheitis virus in allsamples, were negative and since the study method in the present study was ELISA, it isrecommended to investigate the prevalence of infectious laryngotracheitis in native chicken flocks,Laying flocks, broiler breeder and also in broiler flocks in different seasons, with differentdiagnostic methods as well as in other provinces of the country to prevent the spread of the diseaseand extensive economic losses caused by this disease.

کلیدواژه ها:

Infectious laryngotracheitis (ILT) ، Indirect ELISA ، Seroprevalence ، Guilan province.