Evaluation of the effect of soil actinomycetes extracts on thegrowth of pathogenicity of fluconazole-resistant strains of Candidaalbicans

Background and Aim : Candida species are the main cause of fungal infections in humans.Candida albicans is the most virulent and dominant, causing about ۹۰% of all invasive yeastinfections. In recent years, the importance of diseases caused by Candida species has increaseddue to the emergence of resistance to some antifungal drugs such as fluconazole. Therefore,attention has been directed to the screening programs of microorganisms for the production ofantibiotic compounds. Among the microorganisms used to control fungal diseases, Actinomyceteshave been considered due to their wide range of inhibitory activity and high viability. Our aim ofthis study is to find an effective substance from the source of bacteria that is effective againstfluconazole-resistant Candida albicans.Methods : In this study, we took the aqueous extract of Streptomyces isolated from the soil.Streptomyces aqueous extract in the concentration range of ۱.۸ micrograms to ۰.۰۰۰۹ microgramswas effective on Candida albicans isolated from oropharyngeal candidiasis patients by micro brothdilution method. As a control, we treated Candida albicans with fluconazole at a concentration of۶۴ mg/ml.Results : Streptomyces isolated from soil were effective on Candida albicans at the givenconcentrations, and its minimum inhibitory concentration was ۰.۰۰۳۶ ?g. This fungus treated withfluconazole with a concentration of ۶۴ mg/ml had significant growth and was resistant to the drug.Conclusion : Our findings showed that the aqueous extract of Streptomyces isolated from soilaffects Candida albicans while it is resistant to fluconazole, it can be considered a promisingtreatment and more studies should be conducted on it.

کلیدواژه ها:

Candida albicans ، Streptomyces ، candidiasis ، fluconazole