Isolation and identification of nanocellulose producingbacteria from vinegar

Background and Aim : Bacterial cellulose is a biopolymer with unique properties that has manyapplications in all fields from industry to medicine. It has a good potential as wound dressingbecause it provides a moist environment for wound healing and can significantly control woundexudate. Due to its many applications, the production and optimization of nanocellulose fromnative strains has received much attention in recent years.Methods : Native bacterial cellulose producing strains were isolated from ten local vinegarsamples. Bacterial cellulose was produced statically in Hestrin Schramm (HS) medium at ۲۸° Cfor ۷ days. The wet and dry weight of the purified nanolayer were recorded and the water holdingcapacity was determined. The best isolate was selected and identified by different phenotypicmethods and ۱۶S rRNA gene sequencing.Results : Two isolates, namely S۲ and C, were isolated from vinegar samples. Both strains wereGram negative non-motile short rods. The production of BC by the strain S۲ cultivated in HS brothfor ۷ days were ۱۶.۶ g/L while the strain C demonstrated BC production of ۱۰ g/L. The strains S۲and C shared ۹۸.۶۵% an ۱۰۰% similarity to Komagataeibacter oboediens and Komagataeibactersucrofermentans, respectively.Conclusion : Locally isolated strains have showed significant bacterial cellulose productionpotential. Further Studies on the BC production, optimization of conditions and structuremodifications are highly recommended to improve its potential as wound dressing.

کلیدواژه ها:

bacterial nanocellulose ، Gluconacetobacter ، Isolation ، Wound dressing