Molecula detection of probiotic bacteria isolated fromMauremys caspica stool

Background and Aim : In this study, ۵۶ samples of turtles were collected completely randomlyin the cities of Mazandaran province. Due to the role of probiotic bacteria in the intestine andincreasing life expectancy, samples were taken from turtle feces. Probiotic bacteria were analyzedby PCR with global primer.Methods : (Exclusive) was identified. Add one gram of sample prepared with MRS broth in amicrotube with a volume of ۲ ml and ۱ ml of glycerol to the liquid medium and keep at roomtemperature until stable, then ۵۰ Landa samples are taken from the liquid medium and cultured onMRS AGAR linear medium. Data and put it with gas pack C in the JAR and the colonies after ۴۸hoursAnaerobic incubation was calculated.Hot staining colonies were prepared which were usedfor morphological diagnosis, research and probiotic confirmation.Results : A microbial bank was prepared from a sample of isolated bacteria. Probiotic probioticbacteria were observed in Amol, Amirkola, Joybar, Behshahr and Gulogah counties usingmolecular PCR method and Lactobacillus bacteria were observed in Neka, Bahnemir, Sari,Mahmoudabad and Behshahr counties. In samples ۱۸, ۲۱, ۲۵, ۳۸, ۳۹, ۵۴ were Lactobacillus andin samples ۱۰, ۳۶, ۴۴, ۵۲, ۵۶ were pediococcus.Conclusion : According to the results obtained in this study, probiotic bacteria may have beeneffective in increasing the lifespan of Caspian turtles

کلیدواژه ها:

MRS broth ، MRS agar ، gas pack C ، jar ، probiotic