Survey of Metalo beta Lactamase genes, bla IMP۱, INT ۱ inAcinetobacter baumanii isolated from burn patients Zare Hospital ofSari
محل انتشار: بیست و سومین کنگره بین المللی میکروب شناسی ایران
سال انتشار: 1401
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 52
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شناسه ملی سند علمی:
MEDISM23_520
تاریخ نمایه سازی: 16 مهر 1401
چکیده مقاله:
Background and Aim : Acinetobacter baumanii is one of the most important pathogenic bacteriacausing infectious diseases. The aim of this study was to evaluate the antibiotic resistance anddetermine the frequency of β-lactamase and class I integron genes in clinical strains ofAcinetobacter baumanii isolated from burn patients in Zare and BooAli Hospital of sari.Methods : A. baumanii specimens were collected from ۲۰۱۹ to ۲۰۲۱. After culture in a standardmedium, the presence of A. baumanii was confirmed by biochemical tests. Antibacterial sensitivitytest against ciprofloxacin, imipenem, meropenem, Cefepime, ceftazidime, gentamicin, amikacinand colistin was performed using disc diffusion method on the Muller-Hinton agar medium.Finally, polymerase chain reaction was performed to determine the resistance genes.Results : A total of ۱۰۰ A. baumanii were isolated. The results of the pattern of resistance of A.baumanii to the antibiotics tested were as follows: ceftazidime (۹۰%), cefepime (۸۴%),meropenem (۸۱%), imipenem (۷۸%),ciprofloxacin (۷۰%), amikacin (۶۸%), gentamicin (۵۸%),colistin (۱۰%). The study showed that ۳۴% of the isolates produced the metallo-β-lactamases(MBLs) enzyme. Finally, by PCR method, blaIMP, blaVIM and class I integron were identifiedin, ۵۵%, ۴۵% and ۶۰% of these isolates, respectively.Conclusion : This study revealed an increased frequency of MBL-encoding genes (VIM, IMP)and class I integrin in A. baumannii isolates. Also, the results of this study showed that the rate ofresistant to antibiotic among A.baumanii is high. In the present study, the only antibiotic that wassuitable for the treatment of these patients was colistin.
کلیدواژه ها:
نویسندگان
Mohammad Ahanjan
Antimicrobial Resistance Research Center, Mazandaran University of Medical Sciences, Sari, Iran
Behnam Hashemi
Student Research Committee, Mazandaran, University of Medical Science, Sari, Iran
Shahram Divsalar
Zare Hospital Mazandaran University of Medical Sciences, Sari, Iran
Ebrahimm Nemati Hevillee
BooAli Hospital Mazandaran University of Medical Sciences, Sari, Iran