Anti-bacterial and Anti-Quorum Sensing Properties ofDionysia Revolute Boiss against Secondary Bacterial Infections ofCOVID-۱۹ Patients; An in-vitro Study

Background and Aim : Abstract Background and Aim: Today, the use of traditional plantcompounds to kill or interfere with their quorum sensing (QS) mechanisms is considered as analternative approach to control secondary bacterial infections during or after a viral infection. Inthis study, anti-bacterial and anti-quorum sensing effect of Dionysia revolute Boiss against fivesecondary bacterial infections of COVID-۱۹ patients were evaluated.Methods : Materials and Methods: Extraction of the plant compounds was carried out using nhexane,methanol, and ۹۶% ethanol mixed solvent. Bacterial samples were collected fromrespiratory tract fluids among COVID-۱۹ patients and recognized with API kits. Antibacterialactivity of the herbal extract was assessed by disc diffusion method as proposed by the ClinicalLaboratory Standards Institute (CLSL, ۲۰۱۵). Hence, anti-QS activities of this herbal extract at thesub-minimum inhibitory concentration (MIC) were assessed by violacein quantification assay inChromobacterium violaceum CV۰۲۶ biosensor strains in vitro.Results : Results: As it has been indicated in the Results section, a plant extract from ۵۰ to ۰.۳۹mg/ml exposed their antibacterial impacts via hindering the bacterial growth in comparison withcontrols and exhibited anti-QS activities via decreasing the violacein formation in C. violaceumCV۰۲۶ biosensor strain at sub-MIC concentrations (۳.۱ to ۰.۳۹ mg/ml) in vitro.Conclusion : Conclusion : Our study showed that the antimicrobial activities of Dionysia revoluteBoiss could be due to their anti-QS properties. Therefore, this medicinal plant either as a standalonetreatment or in combination with antibiotics could be used as an efficient choice for curingsecondary bacterial infections.

کلیدواژه ها:

Dionysia revolute ، Anti-bacterial ، Anti-quorum sensing ، Secondary bacterialinfections ، SARS COVID-۱۹