The prevalence of ESBLs and biofilm formation in Escherichiacoli isolated from urinary tract infection in Isfahan, Iran

Background and Aim : Background: Uropathogenic Escherichia coli are gram-negative bacillithat most common causes of UTI. This organism has the ability to produce biofilm as an importantvirulence factor that contributes to the stability and recurrence of the disease.There is a need to dothis due to the lack of sufficient information about these resistances in this geographical area andto help increase our knowledge about these genes and their role in creating resistance, as well asthe role of biofilm in increasing resistance.Methods : Methods: ۱۳۹ E. coli were isolated from urine samples. Antibiotic susceptibility ofisolates was determined by disk diffusion method and production of ESBLs confirmed usingDouble disk synergy test method. Molecular detection of the ESBL genes was performed usingPCR. Biofilm formation assay was performed by microtiter plate method.Results : Results: the most effective antibiotics against this bacterium are Nitrofurantoin.Multidrug resistance was observed in ۱۱۹ (۸۵.۶%) isolates. There was phenotypic resistance toESBLs in ۹۳ (۶۶.۹%) isolates. The results of PCR showed that the prevalence of bla-CTX, bla-VEB and bla-TEM were ۴۵(۳۲.۴%), ۸۷(۶۲.۶%) and ۱۰(۷.۲%), respectively. The results of biofilmformation revealed that ۶۵ (۴۶.۸%), ۵۸(۴۱.۷%), ۱۰(۷.۲%), and ۶(۴.۳%) of the isolates had nonbiofilm,weak, moderate, and strong activities, respectively.Conclusion : Conclusions: The high prevalence of ESBLs gene is considered as a risk factor forisolates in this region because it is capable of transmitting to other susceptible bacteria and causingresistance in them. The results of this study showed that biofilm production can increase antibioticresistance.

کلیدواژه ها:

Escherichia coli ، ESBLs ، Biofilm formation ، Antibiotic resistant