Exploitation of two selected immunogenic proteins, BauA andOmpA, for protection against Acinetobacter baumannii infection
محل انتشار: بیست و سومین کنگره بین المللی میکروب شناسی ایران
سال انتشار: 1401
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 223
نسخه کامل این مقاله ارائه نشده است و در دسترس نمی باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
MEDISM23_497
تاریخ نمایه سازی: 16 مهر 1401
چکیده مقاله:
Background and Aim : Acinetobacter baumannii is a hospital opportunistic pathogen, a gramnegativeand non-flagellated bacillus, which is considered a common nosocomial infection withhigh mortality mostly causes sepsis and meningitis. And infection of the urinary tract.Reproduction and persistence of A. baumannii in eukaryotes based on iron uptake functionsincluding siderophore biosynthesis. Iron transfer into the cytosol is mediated by specific membranereceptors that detect iron-siderophore complexes. The expression of this Acinetobactin-mediatediron uptake system is critical for the intracellular growth of A. baumannii. OmpA is the mostabundant membrane protein gram-negative bacteria and is also the major protein of bacterialpathogenesis. The production of new monoclonal antibodies against outer membrane protein A(OmpA) could be considered a potential tool to improve the treatment of A. baumannii infections.A. baumannii is usually resistant to beta-lactams, aminoglycosides, rifampin, andfluoroquinolones. This bacterium has led to the use of new therapies such as vaccines. No vaccineis known for this bacterium, but it is still worth considering. In this study, we used two separatelyselected and recombinant proteins, OmpA and BauA, as vaccine candidates to evaluateimmunogenicity against A. baumannii in a mouse model.Methods : Based on a pre-designed primer from Shahed University Bank, BauA and OmpA genefragments were extracted from the bacterial genome PCR and the clones simulated in pet۲۸ wereexpressed in Escherichia coli BL۲۱(DE۳). The product was analyzed by the SDS-PAGE methodand purified by the Ni_NTA affinity chromatography method. These proteins were injected intoBALB/c mice separately and in combination. The titer of IgG-specific antibody produced againsteach group was determined after the experiment using the indirect ELISA method. Bacterialproteins were then identified by IgG immunoblotting.Results : OmpA and BauA were already reported to raise antibodies against these proteins. Thesame results were obtained. The combination of the two antigens led to significant protectionagainst A. baumannii in comparison to the single antigens.Conclusion : Administration of the combined antigens triggers better protection than singleantigens.
نویسندگان
Motahare Tamehri
Department of Biology, Shahed University, Tehran-Iran
Iraj Rasooli
Molecular Microbiology Research Center and Department of Biology, Shahed University, Tehran-Iran