Isolation and Identification of grapevine endophytic bacteria inwest Azerbaijan province

Background and Aim : Grapevine is one of the most important agricultural products in Iran. WestAzerbaijan province is considered as one of the biggest production centers of grape in Iran, due toits suitable climatic conditions. The aim of this study was to identify grape endophytic bacteria inWest Azarbaijan.Methods : In this study, ۶۷ endophytic bacteria were isolated from the stems and roots ofgrapevines. Then, biochemical properties such as hypersensitive reaction, fluorescent and potatosoft rot test and the ability to produce proteases, amylase and gelatinase enzymes were tested.Eleven bacterial isolates were selected for molecular identification and it was found that theBacillus and Pseudomonas are the most abundant genera.Also, the antifungal effect of endophyticbacteria on three fungal species: Chaetomium globosum, Cytospora chrysosperma and Fusariumsp. was done by dual culture method.Results : According our knowledge, three species of Stenotrophomonas sp., Bosea lathyri andFrigoribacterium faeni are reported for the first time in Iran as grapevine endophytic bacteria.Three isolates of GI۶ (Priestia sp.), GI۴۳ (Pseudomonas kilonensis) and GI۴۵ (Bacillus sp.)showed the most growth inhibitory properties against fungi.Conclusion : Due to the importance of endophytic bacteria, their isolation and identification fromdifferent parts of the country seems necessary, as the result, these bacteria can be used in biologicalcontrol of plant diseases and can be replaced by chemical control.

کلیدواژه ها:

Endophyte ، bacteria ، grape ، biochemical properties ، antifungal effect ، biologicalcontrol