bioinformatic analysis of Nucleocapsid (N) gene in humancoronaviruses Comparing with other animal Coronaviruses

Background and Aim : Due to the recent coronavirus epidemic and the widespread spread ofCOVID-۱۹, the importance of studying coronaviruses, especially in the field of their molecularcharacteristics, became more clear Based on pervious archives, the first disease related to thecoronavirus recorded so far was probably related to animals. However, the most evolutionarystudies focused on S۱ gene, Survey on other gens of coronaviruses such an N gene, that isimportant in immune responses against virus, could be very helpful.Methods : Human, porcine, rodent, avian, bovine, feline, Canine, and bat coronavirus N geneswere collected from the gene bank (NCBI database). After the alignment of N gene of all usedcoronavirus sequences, using Mega X software (version ۱۱), the similarity rate between the N genesequences of human and animal coronaviruses was determined. Then, phylogenetic tree was drawnbased on N gene sequences.Results : According to the results, the rate of nucleotide similarity was shown about ۹۲-۹۵%. Thehighest similarity was between SARS-CoV is WGS-۳۶ and bat coronavirus BM۴۸-۳۱ and thelowest similarity was related to bat coronavirus BM۴۸-۳۱ and another used isolate SARS-Cov.Based on the evolutionary tree, human SARS coronaviruses grouped together with a batcoronaviruses and rodent coronavirus and also three bovine coronaviruses while some other batcoronaviruses were clustered in other group distinct from SARA-Cov.Conclusion : Evolutionary studies of different genes of various coronaviruses specially which hasnotable role in immunity against the viruses could be significant to control the disease and designeffective vaccines. Also, different similarity rates between SARS- Cov isolates and bat coronavirusBM۴۸-۳۱ could be explain the possibility of mutations in the N gene of coronaviruses.

کلیدواژه ها:

coronavirus ، bioinformatics ، N gene