Evaluation the treatment of diarrhea by phage cocktail product

Background and Aim : Diarrhea is one of the most common types of gastrointestinal infections.Salmonella enterica, Shigella flexneri cause diarrhea. The inappropriate use of antibiotics incommon infections such as diarrhea has contributed to antibiotic resistance throughout the world.Alternative treatments such as bacteriophages are suggested. Bacteriophage solely target and killbacteria. Unlike antibiotics, they do not affect the normal bacterial flora, eukaryotic cells. Chitosanas drug delivery system is required to protect phages from being destroyed under the acidicconditions in the stomach. This current research intended to study the construction of a chitosanencapsulated bacteriophage cocktail against bacteria causing diarrhea.Methods : This was investigated using ۶۴ Wistar female rats (۸ weeks old, ۱۸۰ ± ۲۰۰ g) infectedwith Salmonella enterica, and Shigella flexneri. ۰.۵ mL of the phage cocktail was orally gavageddaily. In the placebo group and the positive control group, chitosan and cefixime were orallygavaged daily and the group was not treated. Briefly, the phage cocktail was isolated from sewage.The spot test was performed to determine the lytic activity of the phage cocktail.Results : The results showed that in the treated group with cefixime, weight loss was significantcompared to the chitosan encapsulated bacteriophage cocktail group (p <۰.۰۵). The weight lossfor rats in the non-treated and placebo groups was significantly higher than the group treated withphage (p < ۰.۰۰۱). Positive cultured stools were reduced after ۴ days in the treated group with thebacteriophage cocktail.Conclusion : chitosan encapsulated bacteriophage cocktail treatment of diarrhea was successfullydeveloped.

کلیدواژه ها:

Bacteriophage Cocktail ، Diarrhea ، treatment