Bacteriophage isolation against MDR Enterococus strains

Background and Aim : Enterococci are the normal flora in the human gastrointestinal tract andmany mammals. They are also found in the human oral and vaginal cavities, soil, water, and food.In healthy people the presence of Enterococci usually has no negative effect on the host, but undercertain conditions can cause infection and diseases such as endocarditis, abdominal abscesses,bacteremia and urinary tract infections and play an important role in the distribution of resistancegenes. Enterococci are Facultative anaerobic bacteria, Gram-positive, and catalase-negative thatinclude an important and diverse group of bacteria. Among the species of Enterococci,Enterococcus faecalis and Enterococcus faecium are the main pathogens. With the prevalence ofEnterococci and the increasing use of antibiotics, MDR Enterococci are one of the most commonnosocomial pathogens worldwide. This has led researchers to look at alternative ways to eliminateand control these bacteria. Phage therapy using bacteriophages to treat infection caused bypathogenic bacteria is considered as one of the alternatives to antibiotics. The aim of this studywas to isolate effective bacteriophages against MDR enterococcal strains and to identify them.Methods : After collecting Enterococcus samples from Isfahan hospitals, disk diffusion methodwas used and all samples were resistant to the meropenem, moxifloxacin, vancomycin,streptomycin, gentamicin, and ciprofloxacin.Results : . Microbiological, biochemical, PCR and ۱۶S rRNA gene sequencing methods wasperformed to know specific bacteriophage and bacteri. Bacteriophages were examined for plaqueformation and after enrichment and concentration, plaque was saw ,and it mean bacteriophage and۲ strains of bacteria were foundConclusion : The aim of this study was to isolate effective bacteriophages against MDRenterococcal strains and to identify them. And after gene sequencing two strain of Enterococcusfaecium,E۱ andE۱۱were found and then they were registered in NCBI.

کلیدواژه ها:

Enterococci ، ، Phage therapy ، Bacteriophage