Bacteriophage-derived endolysins as a novel candidate fortreatment of P. aeruginosa infection

Background and Aim : Pseudomonas aeruginosa is a ubiquitous organism which has emerged asa leading cause of hospital-acquired infections. Overuse of antibiotics has significantly expandedthe emergence of multi-drug resistant bacterial infections that can't be treated with multipleantibacterial drugs. While whole phage treatment demonstrated tremendous effects against avariety of P. aeruginosa clinical and general laboratory strains, phage-encoded endolysins havealso shown promising results in external peptidoglycan degradation without the assistance ofphage. Therefore, in this study we aimed to design a product from blending of different endolysinsderived from Pseudomonas phages to be used as a treatment for P. aeruginosa infections.Methods : According to the current literature endolysin Q۸۵۹G۴ demonstrated highest efficacytoward pathogenic P. aeruginosa. The UniProt search was done to find similarity with proteinQ۸۵۹G۴ based on its host. The similar proteins were deposited in PDB and Swiss-Model. The ۳Dstructure checked on PyMOL and the PPI was done in HADDOCK۲.۲.Results : There are ۴۸۰۰۰ proteins similar to endolysin Q۸۵۹G۴ (endolysin of P.aer phage gh.۱)but only ۲۴۶ of identified proteins showed endolysin activity, among which ۱۰ have effect on P.aeruginosa. Forty percent (۴/۱۰) of these phage endolysins had more than ۵۰% identity toendolysin Q۸۵۹G۴ (endolysin of chf-۱۹, CHF.۷ shl۲ and MR۲ phage).Conclusion : The Q۸۵۹G۴ endolysin is similar to endolysins from chf-۱۹, CHF.۷ shl۲ and MR۲phages; therefore blending of these endolysins in the same product could be an appropriatecandidate for treatment of P. aeruginosa infections.

کلیدواژه ها:

Multidrug resistance ، Bacteriophage therapy ، endolysin