evaluating the effect of methanolic extract of Quercus persica onPseudomonas aeroginosa in combination with specific lytic phage

Background and Aim : Introduction: Based on the recent reports of World Health Organization,increased antibiotic resistance prevalence among bacteria represents the greatest challenge tohuman health. antibiotic resistance is rising to dangerously high levels in all parts of the world.New resistance mechanisms are emerging and spreading globally, threatening our ability to treatcommon infectious diseases. Quercus persica is the most important tree species of the Zagros inIran. Iranians use its seed in traditional medicine. Due to the rise of multidrug-resistant infectionsin humans, phage therapy is gaining renewed attention in Western medicine. Despite the increasingnumber of publications focussed on the isolation, characterization and in vitro performance ofdifferent phages, there is still a lack of concise pre-clinical information to guide the application ofphage therapy in clinical practice. Phage Therapy is the therapeutic use of lytic bacteriophages totreat pathogenic bacterial infections.Methods : Method: The present study describes the effect of methanolic extract of Quercus persicaBoiss on Pseudomonas aeroginosa alone and in combination with ۲ specific lytic phage againstthese pathogens. Methanol extracts were prepared by maceration method, filtered and concentratedby rotary evaporator apparatus.Results : Result: MIC value of methanolic extract of Quarcus persica for Pseudomonas aeroginosawas ۱.۲۵ mg /ml . MOI value For lytic Phage was ۱۰۰. The effects were improved by combiningthe extract with phage. In combination extracts with phage,concentrations lower than MIC (forextracts) and less than optimal MOI (for phages) completely inhibited the growth of Pseudomonasaeruginosa.Conclusion : Conclusion: Both phages were effective in preventing from formation andeliminating Pseudomonas aeruginosa biofilm. Combination with methanolic extract of Quarcuspersica enhanced the effect of phages.

کلیدواژه ها:

Bacteriophage ، methanol extract ، Quercus persica ، Pseudomonas aeruginosa