Epidemiological use of nested PCR targeting the QpRSplasmid associated with the chronic form of Q fever in horses of WestAzarbaijan province

سال انتشار: 1401
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 52

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شناسه ملی سند علمی:

MEDISM23_440

تاریخ نمایه سازی: 16 مهر 1401

چکیده مقاله:

Background and Aim : Q fever is a worldwide zoonosis caused by the obligate intracellularpathogen Coxiella burnetii, affecting a broad range of animal hosts including horses. Q fevercauses highly variable symptoms ranging from acute (often self-limited) infection to fatal chronicinfection. The manifestations of chronic Q fever are endocarditis, hepatitis, osteomyelitis, orinfected aortic aneurysms. Most of the isolates found, carry plasmids which genetic studies of C.burnetii strains suggest a critical role in C. burnetii survival. Although the correlation between anisolated plasmid type and the chronic or acute nature of the disease has always been controversial,strains with a QpRS plasmid are associated with chronic Q fever. This study was conducted toinvestigate the prevalence of C. burnetii QpRS plasmid in horses and to assess the potential roleof these animals as reservoirs of infection and transmission.Methods : Nested-PCR assays were performed on ۳۲۰ blood serum samples drawn from horsesin the West Azarbaijan province. In total, ۲۶ (۸.۱%) Q fever-positive samples, based on containingthe IS۱۱۱۱ gene were tested by the Nested- PCR approach to amplify QpRS plasmid segments.Results : Based on these results all the diagnosed Q fever samples were negative for the QpRSplasmid. And horses of west azarbaijan are free of chronic Q fever. The results indicate that theNested PCR method could be suitable for routine diagnosis, gathering new information about theshedding of C. burnetii, and improving the knowledge of contamination routes.Conclusion : Thus, the identification of C. burnetii plasmids may provide important informationfor the differential diagnosis of Q fever and epidemiological investigations.

نویسندگان

Manijeh Tehrani

Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, West Azerbaijan, Iran.

Abdolghaffar Ownagh

Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, West Azerbaijan, Iran.