Evaluating the Effectiveness of Natural Detergent andDisinfectant on Gram-Positive and Gram-Negative Bacteria

Background and Aim : Fruits and Vegetables play an important role in health maintenance andhuman living conditions improvement and their daily consumption can have positive effects onthe prevention of diseases outbreak. It is natural that like any other type of food, they may beexposed to the attack of microbes and cause poisoning and illness in humans. The purpose of thisstudy is evaluating the effectiveness of a detergent and disinfectant, all its components are consistof natural materials and it is a product of Zist Faravard Pars company on two bacteria, Escherichiacoli and Listeria monocytogenes.Methods : In this experimental study, fruit and vegetable samples (Parsley, Cucumber, Celery,Grape by E.coli) and (Tomato, Kiwi, Lettuce, Green plum by L.monocytogenes) were infectedwith a concentration of ۰.۵ McFarland (۱.۵ × ۱۰ to the power of eighth cfu/ml) and wereinvestigated in terms of microbial growth, Then natural detergent and disinfectant was sprayed onthe desired samples and after washing with sterile distilled water, They were investigated andcompared in terms of the growth of bacteria. The minimum inhibitory concentration (MIC) andthe minimum Bactericidal concentration (MBC) of the natural detergent and disinfectant weredetermined using the broth dilution method against the used microorganisms.Results : The results of this study with comparing cultured plates before and after using naturaldetergent and disinfectant showed that the growth of E.coli and L.monocytogenes decreasedsignificantly after using the desired detergent and disinfectant. MIC values for E.coli and L.monocytogenes was determined ۰/۱, ۰/۵ mg/ml to the power of minus one respectively and MBCvalues for E.coli and L. monocytogenes was determined ۰/۰۱, ۰/۰۵ mg/ml to the power of minusone respectively.Conclusion : The desired natural detergent and disinfectant has significant effectiveness ininhibiting the growth of Gram-positive and Gram-negative bacteria used in this study.

کلیدواژه ها:

Natural detergent and disinfectant ، Inhibitory ، Escherichia coli ، Listeriamonocytogenes