Molecular Detection of Brucella spp. in camel population insouthern Kerman Province of Iran

Background and Aim : Brucellosis is a global problem in public health and livestock. Theoccurrence of disease in humans is mainly due to animal reservoirs. Brucellosis disease in camelscan also cause significant economic losses and is very important in terms of public health. Thepresent study was carried out with the aim of determining the status of Brucella infection in thecamel population of South Kerman by molecular methods.Methods : One hundred blood samples with anticoagulant were taken from female camels in thesouth of Kerman province, three months old and older. Then their DNA was extracted. Theirinfection with Brucella bacteria was evaluated by PCR technique using IS۷۱۱ primer.Results : In the current study, out of the hundred blood samples tested, six samples were infectedwith Brucella bacteria. In other words, the prevalence of brucellosis in this research was about sixpercent.Conclusion : Considering the prevalence of this disease in camels and the high population ofcamel herders in the south of Kerman and the large slaughter of this type of animal in theslaughterhouse as well as the consumption of its milk by citizens and villagers in raw and unboiledform, planning to apply more effective care and prevention policies It is necessary to producevaccines, extensive training to prevent disease epidemics, identify and eliminate sick cases, etc.

کلیدواژه ها:

Camel ، Brucella ، PCR