Updated population genetic understanding of Mycobacteriumbovis in the Iranian cattle, a search for major clonal complexesfrequently observed in the world

سال انتشار: 1401
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 63

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شناسه ملی سند علمی:

MEDISM23_394

تاریخ نمایه سازی: 16 مهر 1401

چکیده مقاله:

Background and Aim : Bovine tuberculosis (bTB) is a neglected disease that affects both cattleand humans. Up to now an increasing number of globally important groups, so called clonalcomplexes, of Mycobacterium bovis have been detected and characterized with their spatialdistribution reported ranged from very extensive to limited only to few regions. In ۲۰۱۱ theRDEu۱, the first ever known clonal complex of M. bovis, was shown not to be circulating in Iran.Interrogation of the Iranian M bovis population has been continued in search for other clonalcomplexes including RDEU۲, African ۱ and African ۲. Here we report the most recent findings ofthis investigationMethods : Using reported PCR protocols, the amplification of target regions were performed ongenomic material from as many as ۲۵ M. bovis isolates prepared through plain heating in boilingwaterbath. Amplicons were sized using conventional gel electrophoresis/ imaging and gel imageswere analyzed against standard DNA ladders. MLVA genotyping using ۲۱ loci was also performedto evaluate level of genetic diversity among the selected panel of M bovis isolatesResults : Viewing the sized PCR products, no evidence to confirm presence of any of theaforementioned clonal complexes was trackedConclusion : We assume while there are historical records of the first dairy cattle beingsystematically imported into Iran in ۱۹۲۰s and ۱۹۳۰s as part of the livestock and dairydevelopment projects, these might have had no or little in introducing exotic M. bovis strains intoIran. The related MLVA typing findings and scenarios explaining the observations have been putforwarded and discussed

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نویسندگان

Zahra Esmaeilpour

Department of Veterinary Aerobic Bacteria, Razi Vaccine & Serum Research Institute, Agricultural Research, Education and Extention Organization(AREEO), Karaj, Iran

Keyvan Tadayon

Department of Veterinary Aerobic Bacteria, Razi Vaccine & Serum Research Institute, Agricultural Research, Education and Extention Organization(AREEO), Karaj, Iran