Co-aggregation of Lactobacillus reuteri isolated from yogurtagainst pathogenic Helicobacter pylori for reduction load of H. pylori inhuman

Background and Aim : Lactobacillus reuteri is a well- known probiotic with beneficial effects tohuman health. This species can be found in different organs of the body such as skin, breast,gastrointestinal tract, and urinary tract. Due to its antimicrobial effects, the beneficial effect againstHelicobacter pylori which is a pathogen in the human gastrointestinal tract can be tested.Therefore, the main aim of this study was to determine the antimicrobial properties of L. reuteriagainst H. pylori.Methods : To doing so, different samples of yogurts around Iran were collected, then ۱gr of eachsamples weighted and solved in ۱۰ mL Saline, then ۱۰ mL of this was added to ۹۰ mL of MRSbroth, following that were cultured on MRS agar and incubated to reach the colonies, each ofcolonies were identified and characterized by staining, biochemical and sequencing analysis todetermine the species. After culturing the H. pylori, the co-aggregation and antibacterial effectswere studied.Results : The sequencing results revealed that the isolated species was L. reuteri and coaggregatedstrongly with H. pylori with large numbers of co-aggregation clumps formed at the bottom of thetube and a clear upper suspension. The results of co-aggregation showed that there is ۹۱.۸% coaggregationbetween L. reuteri and H. Pylori.Conclusion : In summary, based on these results it can be found that the L. reuteri which is aprobiotic can be applied in therapeutic application for treatment of disease related to H. pylori ingastrointestinal tract.

کلیدواژه ها:

probiotic ، co-aggregation ، L. reuteri ، H. pylori