Replacing Meal worm in aquatic diet to increase probioticbacteria and reduce disease

Background and Aim : Consumption of meal worm larvae powder can affect the microbial floraof different parts of the fish body, but in this article, the main focus has been on the microbiota ofthe gastrointestinal tract of trout fed with TM larvae powder. Therefore, a better understanding ofhost-symbiotic pathogen transplantation is essential not only for gaining insight into microbialinvolvement in fish diseases, but also for activating new antimicrobial and antimicrobialapproaches to their treatment. The present study aimed to investigate the effects of using differentpercentages of TM larvae powder in the diet of rainbow trout (Oncorhynchus mykiss) onincreasing the amount of beneficial bacteria and probiotic Bacillus safensis.Methods : After adaptation of the fish and feeding with meal worm larvae powder and completionof the feeding period, the fish were randomly separated from ۴ treatments and ۳ replications andprobiotic and beneficial intestinal bacteria were studied and compared with the control group. Inaddition, the effect of these bacteria on growth performance, survival and other factors affectingthe immune system were evaluated.Results : The results showed that Bacillus safensis bacterium had the highest number of beneficialbacteria and probiotics in the gastrointestinal tract of rainbow trout fed with TM powder, whichwas identified by culture, PCR and phylogenetic tree drawing. Data analysis also showed that thenumber of beneficial bacteria of Bacillus safensis for treatments that used TM larvae powder intheir diet was significantly increased compared to the control group. As this increase in treatmentshowed the highest rate of ۴۵%.Conclusion : The gastrointestinal tract of all vertebrates contains a complex set of microorganisms(microbial communities) commonly known as intestinal microbiota. Intestinal microbiota is aresearch area of interest that is universally available to animals, but most studies on thecomposition and function of intestinal microbiota in vertebrates have been performed in mammals.There is relatively little information about fish gut microbiota and its response to nutritional andenvironmental conditions, despite the fact that fish make up almost half of the living vertebratespecies and are of global economic importance.

کلیدواژه ها:

Meal worm ، probiotic ، Bacillus safensis ، Gastrointestinal