A study of prevalence of vacA d genotypes of Helicobacterpylori isolated from patients with gastrointestinal problems in Mashhad

Background and Aim : Helicobacter pylori is a bacterium that resides in the human stomach,which is associated with gastric diseases. The vacA gene is one of the most virulence factors ofH.pylori and associated with gastric pathology. Pathogenesis of vacA depends on polymorphicdiversity within the signal (s), middle (m), intermediate (i), deletion (d) and c-regions.Theseregions show distinct allelic diversity. The present study aimed to investigate the prevalence ofvacA d۱, d۲ genotypes in the H.pylori isolates from patients with gastric disease.Methods : A total of ۱۰۰ patients suffering from gastric diseases were enrolled. The presence ofurease enzyme in the samples was checked by urease test and Detection of H.pylori infection andgenotyping of vacA d region were carried out by PCR.Results : Of the total of ۶۶ H.pylori isolates, ۲۲ contained the d۱ allele and ۲۹ were subtype d۲.d۲ allele was more prevalent among patients with gastric problems and a significant relationshipwas found between this allele and the development of gastritis.Conclusion : Genotyping of vacA d region might be a reliable marker for the identification ofvacA virulent strains that represent a high risk of developing gastric diseases.

کلیدواژه ها:

H.pylori ، VacA gene ، PCR