Background and Aim : C. perfringens is a gram-positive, rod-shaped anaerobe bacterium that isone of the most common pathogens and causes a spectrum of human and veterinary diseases andhas a prolific toxin-producing ability that produce an array of toxins. C. perfringens has fivetoxinotypes (A, B, C, D, E), according to the presence and combination of the four major toxins:alpha, beta, epsilon and iota. Serological methods such as
ELISA are widely used to detectclostridial toxins, usually these methods are used to identify a specific
toxin in intestinal contentsor pure bacterial culture.Methods : A total of ۸۴ C. perfringens isolates which was obtained from the microbial archive ofRazi Kerman Institute from various types that originated from sheep and goats used in this researchthat were identified as C. perfringens by microbiological, biochemical and
toxin typed by PCRmethod.All isolates were evaluated for production of alpha, beta, and epsilon
toxin by BIOXELISA kit and the he frequency and distribution of toxins in different strains were evaluated.Results : Out of ۸۴, ۶۴ isolates showed the presence of at least one
toxin in
ELISA test, ۳۱, andsix isolates were positive for only alpha and only epsilon
toxin respectively, ۱۷, six isolates showpresence of both alpha and epsilon and both alpha and beta toxins respectively furthermore fourisolates positive for the presence of all three toxins, meanwhile, ۲۰ C. perfringens isolates didn’tshow none of the toxins. The results of this study show that type C isolates were the most positivecases of alpha and beta
toxin while type B isolates were the most positive cases of epsilon toxin,which could be related to the pathogenicity of these types in the isolates studiedConclusion : These findings indicated that the presence of a toxin-producing gene in an isolate isnot directly related to the secretion of all the expected toxins in different types by
ELISA test, anddespite the confirmation of the isolates by PCR, in some cases, the desired
toxin is not expressedand produced.